Agrobacterium-Mediated Transformation in Cucumber (Cucumis sativus L.)
نویسندگان
چکیده
Introduction. Cucumber (Cucumis sativus L.) is an important horticultural crop. Its fruits are used for slicing and pickling and juice extraction worldwide. In India, both the fruits and seeds have long been used in the manufacture of traditional medicines that act as skin conditioners, diuretics and body coolants. Thus, cucumber is a potential candidate for an edible vaccine (6). In India, studies on cucumber tissue culture in general and transformation in particular are very limited (1). In the present study, a reliable transformation and regeneration protocol is reported for cv. Green Long, which is widely cultivated in India.
منابع مشابه
In vitro organogenesis and genetic transformation in popular Cucumis sativus L. through Agrobacterium tumefaciens.
The effect of growth regulators and culture conditions on the morphogenetic response of cotyledonary leaf discs was studied in popular cucumber variety (Cucumis sativus cv. Sheetal). Organogenesis was induced directly without any intervening callus phase on Murashige and Skoog medium supplemented with different concentrations of benzyladenine and indole propionic acid. Best results (93%) were o...
متن کاملThe Co-Involvement of Light and Air Temperature in Regulation of Sex Expression in Monoecious Cucumber (Cucumis sativus L.)
The combination effects of light and air temperature, which were expressed as photothermal ratio (PTR), on the growth and sex expression of three monoecious cucumbers (Cucumis sativus L. cv. “Xintaimici”, “Jinyan 3” and “Jinyan 4”) were studied with hydroponics in this research. The results showed that with the increase of PTR, different growth responses were observed in the three cucumber cult...
متن کاملAcquisition of an Agrobacterium Ri plasmid and pathogenicity by other alpha-Proteobacteria in cucumber and tomato crops affected by root mat.
Root mat of cucumbers and tomatoes has previously been shown to be caused by Agrobacterium radiobacter strains harboring a root-inducing Ri plasmid (pRi). Nine other pRi-harboring alpha-Proteobacteria have subsequently been isolated from root mat-infected crops. Fatty acid profiling and partial 16S rRNA sequence analysis identified three of these strains as being in the genus Ochrobactrum, five...
متن کاملHigh-Throughput Sequencing Identifies Novel and Conserved Cucumber (Cucumis sativus L.) microRNAs in Response to Cucumber Green Mottle Mosaic Virus Infection
Seedlings of Cucumis sativus L. (cv. 'Zhongnong 16') were artificially inoculated with Cucumber green mottle mosaic virus (CGMMV) at the three-true-leaf stage. Leaf and flower samples were collected at different time points post-inoculation (10, 30 and 50 d), and processed by high throughput sequencing analysis to identify candidate miRNA sequences. Bioinformatic analysis using screening criter...
متن کاملEthyl Methanesulfonate (EMS)-Mediated Mutagenesis of Cucumber (Cucumis sativus L.)
Ethyl methanesulfonate (EMS) is a stable and effective chemical mutagen. In this study, cucumber (Cucumis sativus L. cv. “Shannong No. 5”) seeds were treated by 1% EMS for 12 h, 24 h and 48 h to optimize EMS mutagenesis and determined median lethal dose of EMS (1% EMS and 24 h) for “Shannong No. 5”. After treated by 1% EMS for 24 h, 541 M1 plants were grown in greenhouse for phenotype investiga...
متن کامل